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“Antimicrobial Susceptibility Testing of Colistin: Evaluation of Standard Broth Microdilution Method for Gram-negative bacilli”
Naila Begum1 , Karvi Agarwal2 , Amit Garg3
1- SENIOR RESIDENT
2- assistant professor , dr.karvi1593@gmail.com
3- professor and head
Abstract:   (216 Views)
Background and Objectives:Colistin is regarded as the final resort for managing infections caused by multi-drug resistance (MDR) gram-negative bacilli (GNB). Minimum inhibitory concentration (MIC) is used to monitor the development of colistin resistance. This study aimed to assess the performance of  Broth Microdilution Method (BMD) against routine Kirby-Bauer disk diffusion (KBDD) and automated BD Phoenix for the detection of in vitro activity of colistin against GNB
Methods: A cross-sectional study was done in the Department of Microbiology, LLRM Medical College, Meerut Uttar Pradesh from September 2023 to January 2024. KBDD method, BMD method  & BD Phoenix (Becton Dickinson, USA) automated system were used in 320 GNB isolated from various clinical samples to detect Colistin susceptibility. MIC determined by BMD method was interpreted according to  Clinical Laboratory Standards Institute (CLSI) 2023 guidelines.
Results: In our study,320 isolates of GNB were identified from the patients with a mean age of 45.34 years. A total of 320 isolates [145(45.31%) Escherichia coli, 124(38.75%) Klebsiella pneumoniae, 32(10.0%) Pseudomonas aeruginosa, and 19(5.93%) Acinetobacter baumannii complex] were tested simultaneously with all three methods for colistin susceptibility. The overall resistance to Colistin amongst GNB was found to be 17.18%  by the gold standard BMD method, 15.31% by BD Phoenix, and 14.37%  by KBDD.
Conclusion: BMD is the most cost-effective, authentic method for routine testing of colistin susceptibility as compared to other methods. The comparative analysis revealed that BMD is superior to other methods in detecting colistin susceptibility emphasizing its potential role in guiding clinicians for treatment decisions
Keywords: Colistin, Drug Resistance, Microbial Sensitivity Tests, Anti-Bacterial Agents
Full-Text [PDF 443 kb]   (67 Downloads)    
Research Article: Research Article | Subject: Microbiology
Received: 2024/03/22 | Accepted: 2025/02/23
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