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Showing 2 results for Mesenchymal Stem Cells
Effect of Mouse Liver Extract on in Vitro Differentiation of Amniotic Membrane Stem Cells into Hepatocyte-Like Cells
Vahide Vahideh Assadollahi , Masoume Jalalvand, Shahrokh Bagheri, Hamed Esmaiel Lashkarian ,
Volume 10, Issue 6 (11-2016)
Abstract

ABSTRACT

          Background and Objective: Multipotent placental amniotic membrane mesenchymal stem cells (MSCs) are capable of differentiating into specialized tissues under different conditions. The aim of this study was to induce differentiation of placental amniotic membrane MSCs from NMRI mouse into hepatocytes using liver extract.

         Methods: Placental amniotic membrane MSCs from a 14-day pregnant female mouse was used in this study. The cells were incubated with trypsin solution, followed by pipetting. The resulting suspension was cultured in 12-well plates. After confirming their mesenchymal nature, differentiation of the aforementioned cells was induced via exposure to 6, 18, 30 and 60 μg/ml of liver extract. On the 16th day of treatment, immunocytochemical reaction for albumin and periodic acid-Schiff (PAS) test were performed for detection of hepatocyte-like cells.

          Results: Change was observed in the shape of differentiating cells from spindle-like shape to polygonal shape. The immunocytochemical reaction of the differentiated cells was positive. PAS staining also confirmed the accumulation of glycogen particles in the aforementioned cells. Concentration of 6 μg/ml liver extract was found as the effective dose for induction of differentiation.

           Conclusion: The findings of this study show that the placental amniotic membrane-derived MSCs of mouse can differentiate in vitro from spindle-like cells to polygonal hepatocyte-like cells with large nuclei and under the influence of the liver.

Keywords: Placental Amniotic Membrane Mesenchymal Stem Cells, Hepatocyte, In Vitro.


Effect of interferon- on the expression of regulatory NLRP6 and NLRP12 genes in human Wharton’s jelly mesenchymal stem cells
Leila Pirdel, Maryam Safajoo, Masoud Maleki,
Volume 19, Issue 5 (9-2025)
Abstract
ABSTARCT
Background and objectives: Mesenchymal stem cells (MSCs) are well known as a major immune modulator. A subgroup of the nucleotide-binding oligomerization domain (NOD) like receptors (NLRs) has been recently found to play an immune/inflammatory regulatory role.
We aimed to analyze and compare the gene expression level of the NOD‐like receptor family pyrin domain containing protein (NLRP), such as NLRP6 and NLRP12, in Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) treated with interferon-gamma (IFN-γ), the pro-inflammatory cytokine, and untreated cells as well.  
Methods: The immunophenotypic characterization of the isolated WJ-MSCs was performed by flow cytometry. Next, they were cultured with or without IFN-g, followed by the comparison of expression level of NLRP6 and NLRP12 genes by using qPCR.
Results: The treatment of cells with IFN-γ indicated a statistically significant increased expression of NLRP12 gene as compared to untreated cells while the expression of NLRP6 was failed to detect in the cells with or without IFN-γ treatment.
Conclusion: The altered expression level of NLRP12 might be suggested its contributory role in the inflammatory regulation mediated by WJ-MSCs in response to the exposure to IFN-g; however, additional studies are needed to validate its role in experimental inflammatory-related disease models.
 
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