Showing 4 results for Electrophoresis
M Mohammadian Yajloo,, A Sahebgadam Lotfi, M Nasroallahzadeh Sabet, N Zhalehjoo, M Amirian, M Biglarzadeh,
Volume 1, Issue 1 (4-2007)
Abstract
Abstract Background & Objective: Alpha-1-antitrypsin (AAT) is the major component of the human plasma alpha-1 globulin proteins and acts as a major inhibitor of proteolytic enzymes, particularly elastase. AAT deficiency is accompanied by lung, liver and other disorders, therefore, AAT is clinically important and its precise evaluation is diagnostically critical. In present study serum AAT was evaluated by three Cellulose Acetate Electrophoresis (CAE), Trypsin Inhibitory Capacity (TIC) and Single Radial Immunodiffusion (SRID) methods and results wene compared. Materials and methods: AAT evaluation was carried out, by CAE, SRID and TIC Methods, on 318 normal sera obtained from volunteer students of Tehran Universities. Results:The results indicated: 34, 84 and 112 samples by TIC, SRID and CAE methods (with reference ranges of 2.1-3.5 mol/min/ml, 126- 226 mg/dl and 2-4.5% respectively) were abnormal 201samples by CAE and TIC were normal and 29 abnomal, 83 sera were normal by TIC and abnormal by CAE five of them were abnormal by TIC and normal by CAE 227 of the samples were normal and 29 abnormal(TIC and SRID) 57 were normal by TIC and abnormal by SRID and seven samples were abnormal by TIC and normal by SRID. Conclusion: Although CAE and alpha-1 globulin band determination are routine in clinical laboratory, they are not reliable in evaluating AAT. SRID sensitivity is more Than CAE and less Than TIC therefore, TIC is recommended as a precise and reliable method for serum AAT evaluation. Key Words: Alpha-1-antitrypsin, Cellulose Acetate Electrophoresis, Single Radial Immunodiffusion, Trypsin Inhibitory Capacity
Hamid Reza Joshaghani , Saeid Parvizi , Khodaberdi Kalavi , Naser Behnampour, Hadi Joshaghani , Nader Hashemi, Sahar Alijanpour,
Volume 9, Issue 5 (11-2015)
Abstract
Abstract
Background and Objective: Normal hemoglobin (Hb) is formed of a heme group and a protein group known as globin. Globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. Cellulose acetate method is a common way to differentiate haemoglobinopathies. Inability to identify the components of Hb low concentrations and incapability to isolate all Hb types are among the disadvantages of this method. The aim of this study was to report the prevalence of hemoglobinopathies in the North of Iran by capillary electrophoresis method.
Methods: All patients with suspected hemoglobinopathies, referred by physicians for electrophoresis, have been studied in a private center in the city of Gorgan, Iran. The level of HbA2, HbA, HbF and other Hb was recorded.
Results: Overall, 725 blood samples were analyzed using the capillary method. HbE was reported in 2 patients, HbH was observed in 2 patients and Hb Barts was reported in 3 patients. Using the capillary method, among patients with the SDG area, only 4 of 38 (10.52%) had HbS and the majority of them (89.48%) had HbD.
Conclusion: HbD is the most common hemoglobinopathy in the North of Iran.
Keywords: Hemoglobinopathy; hemoglobin D; Capillary Electrophoresis; Iran
Imran Ahmed Siddiqui, Sowmya Gayatri C, Swati Suravaram, Bharat Kumar Reddy, Dhanalakshmi A,
Volume 18, Issue 2 (3-2024)
Abstract
Background: ‘M’ proteins or paraproteins refer to immunoglobulins that are produced by clonal plasma cells and are a characteristic feature of monoclonal gammopathies. Routine electrophoresis on agarose gel and immunofixation can be used to detect immunoglobulin paraprotein (M-protein). We aimed to evaluate the performance of agarose gel electrophoresis alone and in combination with immunofixation for detecting serum M-proteins.
Methods: One hundred and twenty-three patients suspected of paraproteinemia were evaluated. Routine serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) protocols were performed. Data from SPE, and SPE-IFE (gel images and electrophoretograms) were collected and reviewed.
Results: 21% cases were confirmed using the SPE-IFE combination, and among them, 33% had positive light chain (λ) only on IFE. Similarly, nine cases with biclonal gammopathy on SPE were characterized by IFE.
Conclusion: IFE can be a confirmatory test in cases where SPE results are not reliable and it can be a complementary test when characterization of the M protein detected on SPE is required.
Dr Amudha Subramaniam, Dr Saranya Raj, Dr Thashreefa Olakara, Dr Jayakumari S, Dr Veronica Preetha Tilak,
Volume 19, Issue 6 (11-2025)
Abstract
Background: Multiple myeloma (MM) is a hematologic malignancy characterized by the abnormal proliferation of plasma cells within the bone marrow. MM arises from monoclonal gammopathy of undetermined significance (MGUS), which can progress to smoldering myeloma and then symptomatic MM The diagnosis of MM relies on Serum Protein Electrophoresis (SPEP), Immunofixation Electrophoresis (IFE), Free Light Chain (FLC) assays. Additionally, Fluorescence In Situ Hybridization (FISH) plays a crucial role in identifying genetic abnormalities that influence the disease course and prognosis. Objective: This study aimed to evaluate the prevalence of electrophoretic and genetic abnormalities among patients referred for serum protein electrophoresis, with a focus on cytogenetic abnormalities detected by FISH in confirmed MM cases.Materials and Methods: Samples received for SPEP from 2017 to 2023 were analysed. Patients with abnormalities on electrophoresis (distortions, M-spikes) underwent further evaluation, including immunofixation, free light chain assays, bone marrow examination, and other hematologic investigations. Confirmed MM cases were referred for FISH analysis to identify common cytogenetic abnormalities.Results: Out of 800 patients with electrophoretic abnormalities, 100 were confirmed to have multiple myeloma. FISH analysis was available for 68 of these cases, detecting cytogenetic abnormalities in 67.6% of patients. The most common abnormalities were IGH break apart (54.5%), followed by p53 deletion (23.5%), t(4;14) (14.7%), t(14;20) (7.4%), monosomy 13 (5.9%), and monosomy 14 (4.4%). Conclusion: A majority of MM patients exhibited abnormalities on FISH, with IGH break-apart being the most frequently detected. The presence of these cytogenetic abnormalities offers valuable prognostic information and may help guide treatment decisions.
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